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anti p stat3  (ABclonal Biotechnology)
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ABclonal Biotechnology anti p stat3
Anti P Stat3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti stat3  (ABclonal Biotechnology)
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Anti Stat3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti p stat3  (ABclonal Biotechnology)
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Rabbit Polyclonal Anti P Stat3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+stat3+antibody/Phospho-STAT3-Y705+Rabbit+mAb/pmc13522776-19-0-4
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stat3 mab  (ABclonal Biotechnology)
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A,B , qRT-PCR of the relative IGF2BP3 mRNA expression in THP-1 cells (A) or iBMDMs (B) stimulated with SeV, IFN-α, or IFN-β for 9 h. C , IB analysis of IGF2BP3 protein levels in THP-1 cells and iBMDMs infected with SeV. D,E , IB analysis of IGF2BP3 protein levels in THP-1 cells pretreated for 2 h with DMSO or fludarabine (D), or DMSO or SC-514 (E), followed by SeV infection and harvest at 0 and 6 h post-infection. F , ChIP–qPCR of STAT1, <t>STAT3,</t> or p65 occupancy at two IGF2BP3 promoter regions in HEK293T cells using IgG as a negative control and histone H3 as a positive control; enrichment is shown relative to IgG. G-I , Relative luciferase activities of the IFN-β (G), ISRE (H), or NF-κB (I) reporters in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by SeV infection for 24 h. J-L , Relative luciferase activities of IFN-β (J), ISRE (K), or NF-κB (L) reporter in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by poly(I:C) stimulation for 24 h. M-O , qRT-PCR of IFNB1 (M), CXCL10 (N), and ISG15 (O) mRNA levels in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. P , IB analysis of phosphorylated and total TBK1, IRF3, and p65 in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. Q , Reporter-based analyses of bioactive IFN-I in supernatants from HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid and collected at the indicated times post-SeV infection, using 2fTGH-ISRE cells. R-T , VSV replication in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by VSV-GFP infection, was assessed by GFP imaging at 24 h (R) and by qRT-PCR (VSV RNA; S) and IB (VSV-GFP; T) at 0, 12, and 24 h post-infection. Scale bars, 100 µm. Data are presented as mean ± SD. Statistical significance was evaluated using an unpaired two-tailed Student’s t test; exact P values are indicated in the panels. Data in C–E, P, and T are representative of three independent experiments with similar results.
Stat3 Mab, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+stat3+antibody/%5BKO+Validated%5D+STAT3+Rabbit+mAb/bio_rxiv__64898__2026__08__01__742171-177-121-123
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phospho stat3 y705  (ABclonal Biotechnology)
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ABclonal Biotechnology phospho stat3 y705
A,B , qRT-PCR of the relative IGF2BP3 mRNA expression in THP-1 cells (A) or iBMDMs (B) stimulated with SeV, IFN-α, or IFN-β for 9 h. C , IB analysis of IGF2BP3 protein levels in THP-1 cells and iBMDMs infected with SeV. D,E , IB analysis of IGF2BP3 protein levels in THP-1 cells pretreated for 2 h with DMSO or fludarabine (D), or DMSO or SC-514 (E), followed by SeV infection and harvest at 0 and 6 h post-infection. F , ChIP–qPCR of STAT1, <t>STAT3,</t> or p65 occupancy at two IGF2BP3 promoter regions in HEK293T cells using IgG as a negative control and histone H3 as a positive control; enrichment is shown relative to IgG. G-I , Relative luciferase activities of the IFN-β (G), ISRE (H), or NF-κB (I) reporters in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by SeV infection for 24 h. J-L , Relative luciferase activities of IFN-β (J), ISRE (K), or NF-κB (L) reporter in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by poly(I:C) stimulation for 24 h. M-O , qRT-PCR of IFNB1 (M), CXCL10 (N), and ISG15 (O) mRNA levels in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. P , IB analysis of phosphorylated and total TBK1, IRF3, and p65 in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. Q , Reporter-based analyses of bioactive IFN-I in supernatants from HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid and collected at the indicated times post-SeV infection, using 2fTGH-ISRE cells. R-T , VSV replication in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by VSV-GFP infection, was assessed by GFP imaging at 24 h (R) and by qRT-PCR (VSV RNA; S) and IB (VSV-GFP; T) at 0, 12, and 24 h post-infection. Scale bars, 100 µm. Data are presented as mean ± SD. Statistical significance was evaluated using an unpaired two-tailed Student’s t test; exact P values are indicated in the panels. Data in C–E, P, and T are representative of three independent experiments with similar results.
Phospho Stat3 Y705, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+stat3+antibody/Phospho-STAT3-Y705+Rabbit+mAb/pmc13447130-147-32-34
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rabbit anti phospho pstat3  (ABclonal Biotechnology)
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A,B , qRT-PCR of the relative IGF2BP3 mRNA expression in THP-1 cells (A) or iBMDMs (B) stimulated with SeV, IFN-α, or IFN-β for 9 h. C , IB analysis of IGF2BP3 protein levels in THP-1 cells and iBMDMs infected with SeV. D,E , IB analysis of IGF2BP3 protein levels in THP-1 cells pretreated for 2 h with DMSO or fludarabine (D), or DMSO or SC-514 (E), followed by SeV infection and harvest at 0 and 6 h post-infection. F , ChIP–qPCR of STAT1, <t>STAT3,</t> or p65 occupancy at two IGF2BP3 promoter regions in HEK293T cells using IgG as a negative control and histone H3 as a positive control; enrichment is shown relative to IgG. G-I , Relative luciferase activities of the IFN-β (G), ISRE (H), or NF-κB (I) reporters in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by SeV infection for 24 h. J-L , Relative luciferase activities of IFN-β (J), ISRE (K), or NF-κB (L) reporter in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by poly(I:C) stimulation for 24 h. M-O , qRT-PCR of IFNB1 (M), CXCL10 (N), and ISG15 (O) mRNA levels in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. P , IB analysis of phosphorylated and total TBK1, IRF3, and p65 in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. Q , Reporter-based analyses of bioactive IFN-I in supernatants from HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid and collected at the indicated times post-SeV infection, using 2fTGH-ISRE cells. R-T , VSV replication in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by VSV-GFP infection, was assessed by GFP imaging at 24 h (R) and by qRT-PCR (VSV RNA; S) and IB (VSV-GFP; T) at 0, 12, and 24 h post-infection. Scale bars, 100 µm. Data are presented as mean ± SD. Statistical significance was evaluated using an unpaired two-tailed Student’s t test; exact P values are indicated in the panels. Data in C–E, P, and T are representative of three independent experiments with similar results.
Rabbit Anti Phospho Pstat3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+stat3+antibody/Phospho-Stat3-S727+Rabbit+pAb/pmc13450646-7-0-4
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rabbit anti stat3  (ABclonal Biotechnology)
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ABclonal Biotechnology rabbit anti stat3
A,B , qRT-PCR of the relative IGF2BP3 mRNA expression in THP-1 cells (A) or iBMDMs (B) stimulated with SeV, IFN-α, or IFN-β for 9 h. C , IB analysis of IGF2BP3 protein levels in THP-1 cells and iBMDMs infected with SeV. D,E , IB analysis of IGF2BP3 protein levels in THP-1 cells pretreated for 2 h with DMSO or fludarabine (D), or DMSO or SC-514 (E), followed by SeV infection and harvest at 0 and 6 h post-infection. F , ChIP–qPCR of STAT1, <t>STAT3,</t> or p65 occupancy at two IGF2BP3 promoter regions in HEK293T cells using IgG as a negative control and histone H3 as a positive control; enrichment is shown relative to IgG. G-I , Relative luciferase activities of the IFN-β (G), ISRE (H), or NF-κB (I) reporters in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by SeV infection for 24 h. J-L , Relative luciferase activities of IFN-β (J), ISRE (K), or NF-κB (L) reporter in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by poly(I:C) stimulation for 24 h. M-O , qRT-PCR of IFNB1 (M), CXCL10 (N), and ISG15 (O) mRNA levels in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. P , IB analysis of phosphorylated and total TBK1, IRF3, and p65 in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. Q , Reporter-based analyses of bioactive IFN-I in supernatants from HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid and collected at the indicated times post-SeV infection, using 2fTGH-ISRE cells. R-T , VSV replication in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by VSV-GFP infection, was assessed by GFP imaging at 24 h (R) and by qRT-PCR (VSV RNA; S) and IB (VSV-GFP; T) at 0, 12, and 24 h post-infection. Scale bars, 100 µm. Data are presented as mean ± SD. Statistical significance was evaluated using an unpaired two-tailed Student’s t test; exact P values are indicated in the panels. Data in C–E, P, and T are representative of three independent experiments with similar results.
Rabbit Anti Stat3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+stat3+antibody/STAT3+Rabbit+pAb/pmc13450646-6-0-4
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stat3  (ABclonal Biotechnology)
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ABclonal Biotechnology stat3
A,B , qRT-PCR of the relative IGF2BP3 mRNA expression in THP-1 cells (A) or iBMDMs (B) stimulated with SeV, IFN-α, or IFN-β for 9 h. C , IB analysis of IGF2BP3 protein levels in THP-1 cells and iBMDMs infected with SeV. D,E , IB analysis of IGF2BP3 protein levels in THP-1 cells pretreated for 2 h with DMSO or fludarabine (D), or DMSO or SC-514 (E), followed by SeV infection and harvest at 0 and 6 h post-infection. F , ChIP–qPCR of STAT1, <t>STAT3,</t> or p65 occupancy at two IGF2BP3 promoter regions in HEK293T cells using IgG as a negative control and histone H3 as a positive control; enrichment is shown relative to IgG. G-I , Relative luciferase activities of the IFN-β (G), ISRE (H), or NF-κB (I) reporters in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by SeV infection for 24 h. J-L , Relative luciferase activities of IFN-β (J), ISRE (K), or NF-κB (L) reporter in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by poly(I:C) stimulation for 24 h. M-O , qRT-PCR of IFNB1 (M), CXCL10 (N), and ISG15 (O) mRNA levels in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. P , IB analysis of phosphorylated and total TBK1, IRF3, and p65 in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. Q , Reporter-based analyses of bioactive IFN-I in supernatants from HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid and collected at the indicated times post-SeV infection, using 2fTGH-ISRE cells. R-T , VSV replication in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by VSV-GFP infection, was assessed by GFP imaging at 24 h (R) and by qRT-PCR (VSV RNA; S) and IB (VSV-GFP; T) at 0, 12, and 24 h post-infection. Scale bars, 100 µm. Data are presented as mean ± SD. Statistical significance was evaluated using an unpaired two-tailed Student’s t test; exact P values are indicated in the panels. Data in C–E, P, and T are representative of three independent experiments with similar results.
Stat3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+stat3+antibody/%5BKO+Validated%5D+STAT3+Rabbit+pAb/pm42455237-178-38-41
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transcription 3 stat3  (ABclonal Biotechnology)
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ABclonal Biotechnology transcription 3 stat3
A,B , qRT-PCR of the relative IGF2BP3 mRNA expression in THP-1 cells (A) or iBMDMs (B) stimulated with SeV, IFN-α, or IFN-β for 9 h. C , IB analysis of IGF2BP3 protein levels in THP-1 cells and iBMDMs infected with SeV. D,E , IB analysis of IGF2BP3 protein levels in THP-1 cells pretreated for 2 h with DMSO or fludarabine (D), or DMSO or SC-514 (E), followed by SeV infection and harvest at 0 and 6 h post-infection. F , ChIP–qPCR of STAT1, <t>STAT3,</t> or p65 occupancy at two IGF2BP3 promoter regions in HEK293T cells using IgG as a negative control and histone H3 as a positive control; enrichment is shown relative to IgG. G-I , Relative luciferase activities of the IFN-β (G), ISRE (H), or NF-κB (I) reporters in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by SeV infection for 24 h. J-L , Relative luciferase activities of IFN-β (J), ISRE (K), or NF-κB (L) reporter in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by poly(I:C) stimulation for 24 h. M-O , qRT-PCR of IFNB1 (M), CXCL10 (N), and ISG15 (O) mRNA levels in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. P , IB analysis of phosphorylated and total TBK1, IRF3, and p65 in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. Q , Reporter-based analyses of bioactive IFN-I in supernatants from HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid and collected at the indicated times post-SeV infection, using 2fTGH-ISRE cells. R-T , VSV replication in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by VSV-GFP infection, was assessed by GFP imaging at 24 h (R) and by qRT-PCR (VSV RNA; S) and IB (VSV-GFP; T) at 0, 12, and 24 h post-infection. Scale bars, 100 µm. Data are presented as mean ± SD. Statistical significance was evaluated using an unpaired two-tailed Student’s t test; exact P values are indicated in the panels. Data in C–E, P, and T are representative of three independent experiments with similar results.
Transcription 3 Stat3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transcription 3 stat3 - by Bioz Stars, 2026-10
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p stat3  (ABclonal Biotechnology)
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ABclonal Biotechnology p stat3
A,B , qRT-PCR of the relative IGF2BP3 mRNA expression in THP-1 cells (A) or iBMDMs (B) stimulated with SeV, IFN-α, or IFN-β for 9 h. C , IB analysis of IGF2BP3 protein levels in THP-1 cells and iBMDMs infected with SeV. D,E , IB analysis of IGF2BP3 protein levels in THP-1 cells pretreated for 2 h with DMSO or fludarabine (D), or DMSO or SC-514 (E), followed by SeV infection and harvest at 0 and 6 h post-infection. F , ChIP–qPCR of STAT1, <t>STAT3,</t> or p65 occupancy at two IGF2BP3 promoter regions in HEK293T cells using IgG as a negative control and histone H3 as a positive control; enrichment is shown relative to IgG. G-I , Relative luciferase activities of the IFN-β (G), ISRE (H), or NF-κB (I) reporters in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by SeV infection for 24 h. J-L , Relative luciferase activities of IFN-β (J), ISRE (K), or NF-κB (L) reporter in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by poly(I:C) stimulation for 24 h. M-O , qRT-PCR of IFNB1 (M), CXCL10 (N), and ISG15 (O) mRNA levels in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. P , IB analysis of phosphorylated and total TBK1, IRF3, and p65 in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. Q , Reporter-based analyses of bioactive IFN-I in supernatants from HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid and collected at the indicated times post-SeV infection, using 2fTGH-ISRE cells. R-T , VSV replication in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by VSV-GFP infection, was assessed by GFP imaging at 24 h (R) and by qRT-PCR (VSV RNA; S) and IB (VSV-GFP; T) at 0, 12, and 24 h post-infection. Scale bars, 100 µm. Data are presented as mean ± SD. Statistical significance was evaluated using an unpaired two-tailed Student’s t test; exact P values are indicated in the panels. Data in C–E, P, and T are representative of three independent experiments with similar results.
P Stat3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Cell Reports Medicine

Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

doi: 10.1016/j.xcrm.2026.102937

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal anti-p-STAT3 , ABclonal , Cat# AP0705; RRID: AB_2863810.

Techniques: Recombinant, Lysis, Hydroxyproline Assay, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Staining, Software

A,B , qRT-PCR of the relative IGF2BP3 mRNA expression in THP-1 cells (A) or iBMDMs (B) stimulated with SeV, IFN-α, or IFN-β for 9 h. C , IB analysis of IGF2BP3 protein levels in THP-1 cells and iBMDMs infected with SeV. D,E , IB analysis of IGF2BP3 protein levels in THP-1 cells pretreated for 2 h with DMSO or fludarabine (D), or DMSO or SC-514 (E), followed by SeV infection and harvest at 0 and 6 h post-infection. F , ChIP–qPCR of STAT1, STAT3, or p65 occupancy at two IGF2BP3 promoter regions in HEK293T cells using IgG as a negative control and histone H3 as a positive control; enrichment is shown relative to IgG. G-I , Relative luciferase activities of the IFN-β (G), ISRE (H), or NF-κB (I) reporters in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by SeV infection for 24 h. J-L , Relative luciferase activities of IFN-β (J), ISRE (K), or NF-κB (L) reporter in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by poly(I:C) stimulation for 24 h. M-O , qRT-PCR of IFNB1 (M), CXCL10 (N), and ISG15 (O) mRNA levels in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. P , IB analysis of phosphorylated and total TBK1, IRF3, and p65 in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. Q , Reporter-based analyses of bioactive IFN-I in supernatants from HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid and collected at the indicated times post-SeV infection, using 2fTGH-ISRE cells. R-T , VSV replication in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by VSV-GFP infection, was assessed by GFP imaging at 24 h (R) and by qRT-PCR (VSV RNA; S) and IB (VSV-GFP; T) at 0, 12, and 24 h post-infection. Scale bars, 100 µm. Data are presented as mean ± SD. Statistical significance was evaluated using an unpaired two-tailed Student’s t test; exact P values are indicated in the panels. Data in C–E, P, and T are representative of three independent experiments with similar results.

Journal: bioRxiv

Article Title: IGF2BP3 amplifies antiviral innate immunity with implications for autoimmune diseases

doi: 10.64898/2026.08.01.742171

Figure Lengend Snippet: A,B , qRT-PCR of the relative IGF2BP3 mRNA expression in THP-1 cells (A) or iBMDMs (B) stimulated with SeV, IFN-α, or IFN-β for 9 h. C , IB analysis of IGF2BP3 protein levels in THP-1 cells and iBMDMs infected with SeV. D,E , IB analysis of IGF2BP3 protein levels in THP-1 cells pretreated for 2 h with DMSO or fludarabine (D), or DMSO or SC-514 (E), followed by SeV infection and harvest at 0 and 6 h post-infection. F , ChIP–qPCR of STAT1, STAT3, or p65 occupancy at two IGF2BP3 promoter regions in HEK293T cells using IgG as a negative control and histone H3 as a positive control; enrichment is shown relative to IgG. G-I , Relative luciferase activities of the IFN-β (G), ISRE (H), or NF-κB (I) reporters in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by SeV infection for 24 h. J-L , Relative luciferase activities of IFN-β (J), ISRE (K), or NF-κB (L) reporter in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by poly(I:C) stimulation for 24 h. M-O , qRT-PCR of IFNB1 (M), CXCL10 (N), and ISG15 (O) mRNA levels in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. P , IB analysis of phosphorylated and total TBK1, IRF3, and p65 in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, at the indicated time points following SeV infection. Q , Reporter-based analyses of bioactive IFN-I in supernatants from HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid and collected at the indicated times post-SeV infection, using 2fTGH-ISRE cells. R-T , VSV replication in HEK293T cells transfected with empty vector or an IGF2BP3 expression plasmid, followed by VSV-GFP infection, was assessed by GFP imaging at 24 h (R) and by qRT-PCR (VSV RNA; S) and IB (VSV-GFP; T) at 0, 12, and 24 h post-infection. Scale bars, 100 µm. Data are presented as mean ± SD. Statistical significance was evaluated using an unpaired two-tailed Student’s t test; exact P values are indicated in the panels. Data in C–E, P, and T are representative of three independent experiments with similar results.

Article Snippet: Primary antibodies used in this study were: GAPDH (mouse mAb; Abmart, M20006S), β-actin (mouse mAb; Proteintech, 66009-1-Ig), β-tubulin (mouse mAb; Abmart, M30109S), Flag tag (mouse mAb; MBL, M185-3L), HA tag (mouse mAb; Abmart, M20003S), GFP (mouse pAb; MBL, MBL598), IGF2BP3 (rabbit pAb; Proteintech, 14642-1-AP), IGF2BP3 (mouse mAb; Santa Cruz, sc-390639), m 6 A (mouse mAb; Proteintech, 68055-1-Ig), NF-κB p65 (rabbit mAb; Cell Signaling Technology, 8242S), phospho-NF-κB p65 (Ser536) (rabbit mAb; Cell Signaling Technology, 3033S), G3BP1 (rabbit pAb; Proteintech, 13057-2-AP), eIF4E (rabbit mAb; STARTER, S0B6141), PABP1 (rabbit pAb; STARTER, S0B1354), TBK1 (rabbit pAb; Proteintech, 28397-1-AP), phospho-TBK1 (Ser172) (rabbit mAb; Cell Signaling Technology, 5483S), IRF3 (rabbit pAb; Proteintech, 11312-1-AP), phospho-IRF3 (Ser396) (rabbit pAb; Cell Signaling Technology, 4947S), Histone H3 (mouse mAb; Beyotime, Cat#AF0009), STAT3 mAb (ABclonal, A19566), STAT1 mAb (Cell Signaling Technology, 14995S), mouse IgG (Sigma-Aldrich, I5381), rabbit IgG (Sigma-Aldrich, I5006).

Techniques: Quantitative RT-PCR, Expressing, Infection, ChIP-qPCR, Negative Control, Positive Control, Luciferase, Transfection, Plasmid Preparation, Imaging, Two Tailed Test